Defensin beta 4 antibody (Biotin)

Catalog No. ABIN5690601

This Rabbit Polyclonal antibody specifically detects Defensin beta 4 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Defensin beta 4 antibody (Biotin) (ABIN5690601)

Target: Defensin beta 4 (DEFB4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Defensin beta 4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Defensin beta 4 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-4 (Human Beta Defensin-4). hBD-4 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hBD-4 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-4.

Sandwich
To detect hBD-4 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BD-4 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-4.

Western Blot:
To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: BD-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Defensin beta 4

Target: Defensin beta 4 (DEFB4)

Alternative Name: BD-4

Gene ID: 140596

UniProt: Q8WTQ1